FIGURE 1.

A, Study design. Animals were housed before planned procedures. Prior to kidney recovery, a WI time of 30 minutes was applied. Renal grafts were either preserved with NEVKP (A) or SCS (B). After preservation and graft transplantation, pigs were recovered and followed up for 10 days. B, Schematic of the NEVKP circuit. The circuit consists of neonatal cardiopulmonary bypass technology. The perfusion solution is collected in the venous reservoir. A centrifugal pump propels the solution into the oxygenator, where it is enriched with oxygen and warmed to 37°C. After passing the arterial filter, the perfusate is driven with a pressure of 65 mm Hg through the renal artery into the graft located in the customized double-walled kidney chamber. The venous outflow (0-3 mm Hg) leads the perfusate back into the venous reservoir. Syringe and infusion pumps secure the supply with additional compounds. The urine is collected throughout the perfusion. Control panel and DMS indicate and record perfusion parameters continuously. DMS, Data Management System.