Table 1.
Diet formulation and analyzed composition of diets, DM basis
| Treatment1 | |||
|---|---|---|---|
| 20 °C | 45 °C | 100 °C | |
| Diet formulation | |||
| Corn | 79.2 | 79.2 | 79.2 |
| Soybean meal | 8.2 | 8.2 | 8.2 |
| Bovine spray-dried plasma | 8.5 | 8.5 | 8.5 |
| Soybean oil | 1.8 | 1.8 | 1.8 |
| Limestone | 1.2 | 1.2 | 1.2 |
| dl-Methionine | 0.11 | 0.11 | 0.11 |
| l-Lysine·HCl | 0.22 | 0.22 | 0.22 |
| Mineral premix2 | 0.14 | 0.14 | 0.14 |
| Vitamin premix3 | 0.06 | 0.06 | 0.06 |
| Titanium dioxide4 | 0.44 | 0.44 | 0.44 |
| Analyzed composition | |||
| DM, % | 86.9 | 86.8 | 87.2 |
| Ash, % | 4.7 | 4.4 | 4.6 |
| Protein, % | 23.5 | 22.1 | 22.5 |
| Gross energy, % | 4,562 | 4,570 | 4,564 |
| Fat, % | 6.1 | 4.9 | 4.6 |
| Carbonyl in spray-dried plasma5, nmol/mg | 2.7 | 5.2 | 8.1 |
| Carbonyl in final mixed diet5, nmol/mg | 6.2 | 7.3 | 13.5 |
1Difference in dietary treatments were created by either no heat treatment to spray-dried bovine plasma (20 °C), heating plasma at 45 °C for 3 d (45 °C), or heating at 100 °C for 3 d (100 °C).
2Mineral premix supplied per kg of complete diet: 9 ppm Cu, 120 ppm Fe, 120 ppm Zn, 7 ppm Mn, 0.2 ppm I, and 0.2 ppm Se.
3Vitamin premix supplied per kg of complete diet: 2,143 IU vitamin A, 245 IU vitamin D3, 17.5 IU vitamin E, 1.1 IU vitamin K, 3.9 mg riboflavin, 19.6 mg niacin, 9.5 mg pantothenic acid, and 18 μg vitamin B12.
4Titanium dioxide was used as an indigestible marker for determining the apparent total tract digestibility.
5Carbonyls were analyzed as a measure of protein oxidation in the plasma after heating as well as in a complete diet after mixing. Values are reported as nmol of carbonyl/mg of protein.