Skip to main content
. 2020 May 15;6(20):eaax0317. doi: 10.1126/sciadv.aax0317

Fig. 3. Vinculin is required for higher forces per integrin.

Fig. 3

(A) Ensemble FRET maps for WT and vin−/− MEFs transfected with eGFP-paxillin and seeded on coverslips functionalized with MTSlow and MTShigh sensors. Scale bar, 10 μm. (B) Total integrated traction per cell for forces <7 pN measured with MTSlow. Open circles indicate the mean value. (WT: 96 cells, mean: 3.6 nN; vin−/−: 89 cells, mean: 4.4 nN.) (C) Total integrated traction per cell for forces between 7 and 11 pN measured with MTShigh. Open circles indicate the mean. (WT: 71 cells, mean: 8.7 nN; vin−/−: 99 cells, mean: 1.5 nN.) (D) Histograms of the single-molecule load measurements for WT and vin−/− MEFs measured for cells adhering to MTSlow for sensors outside adhesions (left) and within adhesions (right). ***P < 0.001 using two-sided Wilcoxon rank sum test.