Fig. 2. CPEB2 KO females display aberrant Sca1⁺ ductal luminal progenitors.

(A) Representative fluorescence-activated cell sorting (FACS) plots gated on luminal cells depicting luminal subpopulations: ductal differentiated (DD; Sca1⁺CD49b⁻), ductal progenitors (DPs; Sca1⁺CD49b⁺), and alveolar progenitors (APs; Sca1⁻CD49b⁺). (B) Representative FACS plots for Sca1 gated on luminal cells. FSC-W, forward scatter width. (C) Ratio of the percentage of Sca1^high and Sca1^low populations in luminal cells (n = 10). Statistics were determined using the Mann-Whitney test, **P < 0.01. (D) Quantification of the percentage of CD49b⁺ cells gated on luminal cells (n = 17). Statistics were determined using the Mann-Whitney test, *P < 0.05. (E) Quantification of the luminal subpopulations as in (A). Statistics were determined using two-way ANOVA, ***P < 0.001 (n = 17). (F) Preranked GSEA graphical output for the enrichment in DP^KO versus DP^WT cells of the up-regulated genes in the “WT progenitor signature” generated by P value (n = 181, see Methods). FDR q ≤ 0.0001. FDR, false discovery rate; NES, normalized enrichment score. (G) Expression of luminal progenitor markers in DP^WT and DP^KO cells. (H) Expression of luminal progenitor markers in AP^WT and AP^KO cells. (I) Representative images of organoids from sorted DP^WT and DP^KO cells and automatic quantification of the number of organoids from sorted DD or DP cells. Scale bars, 100 μm. Statistics were determined using two-way ANOVA, **P < 0.01.