Figure 4. Flow reduces steady-state inward current through a PKD2-mediated, Ca2+ influx-dependent mechanism in voltage-clamped mesenteric artery endothelial cells.
(A) Original recordings of steady-state current modulation by flow (10 ml/min) and effect of removing bath Ca2+ at −60 mV in endothelial cells from Pkd2fl/fl and Pkd2 ecKO mice. (B) Mean data for flow-induced transient inward current. n = 9 for Pkd2fl/fl and n = 10 for Pkd2 ecKO. * indicates p<0.05 versus Pkd2fl/fl.(C) Mean data for steady-state currents in the presence and absence of flow and in the presence and absence of extracellular Ca2+ (Pkd2fl/fl: static + Ca2+, n = 9; static with zero Ca2+, n = 6; flow + Ca2+, n = 9; flow with zero Ca2+, n = 9 and Pkd2 ecKO: static + Ca2+, n = 9; static with zero Ca2+, n = 15; flow + Ca2+, n = 8; flow with zero Ca2+, n = 8). *p<0.05 versus static + Ca2+ conditions in the same genotype, and indicates p<0.05 vs Pkd2fl/fl under the same condition, # p<0.05 versus flow + Ca2+ in the same genotype.