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. 2020 May 4;9:e56655. doi: 10.7554/eLife.56655

Figure 5. Flow-mediated PKD2 channel activation stimulates SK/IK channels in mesenteric artery endothelial cells, leading to vasodilation.

Figure 5.

(A) Original recordings of steady-state current modulation by flow (10 ml/min) and flow plus apamin/Tram-34 (300 nM of each) at −60 mV in mesenteric artery ECs from Pkd2fl/fl and Pkd2 ecKO mice. (B) Mean data (Pkd2fl/fl: static, n = 8; flow, n = 8; flow + apamin/Tram-34, n = 7. Pkd2 ecKO: static, n = 9, flow, n = 10; flow + apamin/Tram-34, n = 9). * indicates p<0.05 versus static in the same genotype and p<0.05 vs Pkd2fl/fl in the same conditions. # p<0.05 versus flow in the same genotype. (C) Original recordings of steady-state current modulation by apamin/Tram-34 (300 nM of each) in the absence of flow at −60 mV in ECs from Pkd2fl/fl and Pkd2 ecKO mice. (D) Mean data comparing responses to apamin/Tram-34 in static and flow conditions at −60 mV (Pkd2fl/fl: static, n = 6; flow, n = 7. Pkd2 ecKO: static, n = 6; flow, n = 9). *p<0.05 versus static control. # p<0.05 versus static + apamin/Tram-34 in the same genotype. and indicates p<0.05 for Pkd2 ecKO vs Pkd2fl/fl in the same condition.