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. 2020 May 4;9:e56655. doi: 10.7554/eLife.56655

Figure 7. Flow-mediated PKD2 channel activation in ECs stimulates eNOS serine 1176 phosphorylation, leading to vasodilation.

Figure 7.

(A) Original Western blots illustrating effects of flow (15 dyn/cm2) and Pkd2 ecKO on p-eNOS (S1176) and total eNOS proteins in Pkd2fl/fl and Pkd2 ecKO mesenteric arteries. (B) Mean data for flow-induced change (Δ) in proteins. n = 5 for Pkd2fl/fl. n = 4 for Pkd2 ecKO. * indicates p<0.05 versus static. # indicates p<0.05 versus same protein in Pkd2fl/fl. (C) Representative traces demonstrating flow (15 dyn/cm2)-mediated vasodilation in pressurized (80 mmHg) mesenteric arteries of Pkd2fl/fl and Pkd2 ecKO mice in the presence and absence of L-NNA (10 µM). (D) Mean data. n = 10 for Pkd2fl/fl. n = 5 for Pkd2 ecKO. * indicates p<0.05 versus Pkd2fl/fl in the same condition. # indicates p<0.05 versus flow in the absence of L-NNA (10 µM) in the same genotype.