Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. musculus) | Pkd2fl/fl | Baltimore PKD Core Center | PMID:20862291 | Mice with Pkd2 gene flanked by loxP regions. |
| Strain, strain background (M. musculus) | Cdh5(PAC)-creERT2 | Cancer Research UK | RRID:MGI:3848984 | Mice with tamoxifen-inducible Cre recombinase that is expressed specifically in endothelial cells. |
| Strain, strain background (M. musculus) | Pkd2fl/fl: Cdh5(PAC)-creERT2 | This paper | Mouse line created in-house by mating Pkd2fl/fl with Cdh5(PAC)-creERT2. Mice with inducible endothelial cell-specific deletion of PKD2. | |
| Antibody | Anti-PKD2 (rabbit polyclonal) |
Baltimore PKD Core | Rabbit mAB 3374 CT-14/4 | IF 1:200 dilution |
| Antibody | Anti-PKD2 (mouse monoclonal) | Santa Cruz | Cat# sc-47734 RRID:AB_672380 |
WB 1:100 dilution |
| Antibody | Anti-PKD1 (mouse monoclonal) | Santa Cruz | Cat# sc-28331 RRID:AB_672377 |
WB 1:100 dilution |
| Antibody | Anti-Piezo1 (rabbit polyclonal) | Proteintech | Cat 15939–1-AP. | WB 1:100 dilution |
| Antibody | Anti-SK3 antibody | Abcam | Cat# ab28631 RRID:AB_775888 |
WB 1:100 dilution |
| Antibody | Anti-IK1 Antibody (D-5) (mouse monoclonal) | Santa Cruz | Cat# sc-365265 RRID:AB_10841432 |
WB 1:100 dilution |
| Antibody | Anti-eNOS (mouse monoclonal) | Abcam | Cat# ab76198 RRID:AB_1310183 |
WB 1:100 dilution |
| Antibody | Anti-p-eNOS (rabbit polyclonal) | Cell signaling Technology | Cat# 9571 RRID:AB_329837 |
WB 1:100 dilution |
| Antibody | Anti-GPR68 | NOVUS Biologicals | Cat# NBP2-32747 | WB 1:100 dilution |
| Antibody | Anti-TRPV4 (clone 1B2.6) (mouse monoclonal) |
Millipore Sigma | Cat# MABS466 | WB 1:100 dilution |
| Antibody | Anti-Actin (mouse monoclonal) | Millipore Sigma | Cat# MAB1501 RRID:AB_2223041 |
WB 1:5000 dilution |
| Antibody | Alexa 555 secondary antibodies (anti rabbit and anti mouse) | Thermo Fisher | Cat# A-21429 (RRID:AB_141761) and # A-31570 (RRID:AB_2536180) |
IF 1:400 dilution |
| Antibody | Alexa 488 secondary antibodies (anti rat) | Thermo Fisher | Cat# A-21470 RRID:AB_2535873 |
IF 1:400 dilution |
| Other | Nuclear staining (DAPI) | Thermo Fisher | Cat# 3571 RRID:AB_2307445 |
IF 1:1000 dilution |