Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene Mus musculus | NPC1 | PMID:27551080 | ||
| Gene (Homo sapiens) | NPC1L1 | PMID:27075173 | ||
| Gene (Homo sapiens) | LAMP1 | PMID:27664420 | ||
| Cell line (Homo sapiens) | HeLa NPC1 KO | PMID:26578804 | ||
| Cell line (Homo sapiens) | HEK293T | ATCC | ||
| Cell line (Homo sapiens) | 293F | ATCC | ||
| Cell line (Spodoptera frugiperda) | SF9 | ATCC | ||
| Transfected construct Mus musculus | NPC1-eGFP | PMID:27551080 | ||
| Transfected construct (Homo sapiens) | NPC1L1-eGFP | PMID:27075173 | ||
| Transfected construct (Homo sapiens) | LAMP1-eGFP | PMID:27664420 | ||
| Transfected construct (Homo sapiens) | pFastBac-NPC1L1-wt-N-terminal domain | PMID:27075173 | ||
| Transfected construct (Homo sapiens) | pCMV-FLAG-His6-NPC1L1-MLD | PMID:27075173 | ||
| Antibody | Chicken polyclonal anti-GFP | Life technologies | 1:1000 | |
| Antibody | IRDye 680RD polyclonal Donkey anti-chicken | LI-COR | 926–68075 | 1:10000 |
| Antibody | IRDye 800CW streptavidin | LI-COR | 926–32230 | 1:10000 |
| Antibody | Mouse monoclonal anti-GFP | NeuroMab | N86-38 | 1:1000 |
| Antibody | rabbit polyclonal anti-LAMP1 | Novus | NB120-19294 | 1:1000 |
| Antibody | Alexa Fluor 488 polyclonal Goat anti-mouse | Life Technologies | A-11001 | 1:2000 |
| Antibody | Alexa Fluor 568 polyclonal Goat anti-rabbit | Life Technologies | A-11011 | 1:2000 |
| Recombinant DNA reagent | 293 fectin | Invitrogen | ||
| Recombinant DNA reagent | polyethyleneimine | SIGMA-Aldrich | ||
| Recombinant DNA reagent | Lipofectamine 3000 | Life Technologies | Cat #A10262 | |
| Chemical compound, drug | Ezetimibe | Santa Cruz Biotechnology | sc-205690 | |
| Software, algorithm | Flowjo | https://www.flowjo.com/ | ||
| Software, algorithm | FIJI | PMID:22743772, doi:10.1038/nmeth.2019 |