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. 2020 May 1;9:e53895. doi: 10.7554/eLife.53895

Table 1. List of primer pairs used for qPCR or genotyping analysis in this study.

Name Forward Reverse Usage
Axin2 GCAAGTCCAAGCCCCATA CGGCTGACTCGTTCTCCT qPCR
Rspo2 GCCAGGCAAAAGACACAATACCA CAATCAGGCTTCCGCCTCTTCTTC qPCR
Rspo3 TCAAAGGGAGAGCGAGGA CAGGAGGAGGAGCTTGTTTCC qPCR
Rspo4 GCTGTTTCAGGCCAGGGACTTC TTGTGTATGCAGGGGACTCCA qPCR
Sdha TGTTCAGTTCCACCCCACA TCTCCACGACACCCTTCTG pPCR
Wnt4 ACTGGACTCCCTCCCTGTCT TGCCCTTGTCACTGCAAA qPCR
Ctnnb1 AAGGTAGAGTGATGAAAGTTGTT CACCATGTCCTCTGTCTATTC Genotyping
Cre CCCACCGTCAGGTACGTGAGATAT CGCGGTCTGGCAGGTAAAAACTAT Genotyping
Foxd1 Wt CTCCTCCGTGTCCTCGTC TCTGGTCCAAGAATCCGAAG Genotyping
Foxd1 Cre GGGAGGATTGGGAAGACAAT TCTGGTCCAAGAATCCGAAG Genotyping
Lgr4 Wt and Ko TGTGTTTTGGCTTGCTTGAC AGTCTGCTCCCCTACCACT Genotyping
Lgr4 Wt TGCAACCCTAGAAGGGAAAA CTCACAGGTGCTTGGGTGAAG Genotyping
Lgr4 Ko GCCTGCATTACCGGTCGATGCAACGA CTCACAGGTGCTTGGGTGAAG Genotyping
Lgr5 Wt ACATGCTCCTGTCCTTGCT GTAGGAGGTGAAGACGCTGA Genotyping
Lgr5 Ko CACTGCATTCTAGTTGTGG CGGTGCCCGCAGGCGAG Genotyping
Lgr6 Wt CGCTCGCCCGTCTGAGC GCGTCCAGGGTCCGCAGGG Genotyping
Lgr6 Ko CGCTCGCCCGTCTGAGC CCTGGACGTAGCCTTCGGGC Genotyping
Rspo1Ko ATCCAGGGGTCCCTCTTGATC AATATCGCGGCTCATTCGAGG Genotyping
Rspo1Wt ATCCAGGGGTCCCTCTTGATC TTGAGGCAACCGTTGACTTC Genotyping
Rspo3flox CTTCAACTTGAAGGTGCTTTACC CCAGGAATGTACAACAGGATCCTCTC Genotyping