Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Danio rerio) | anxa2a | ensemble: ENSDARG00000003216 | ||
| Gene (Danio rerio) | aqp1a1 | ensemble: ENSDARG00000023713 | ||
| Gene (Danio rerio) | aqp8a1 | ensemble: ENSDARG00000045141 | ||
| Gene (Danio rerio) | cx43.4 | ensemble: ENSDARG00000007099 | ||
| Gene (Danio rerio) | esama | ensemble: ENSDARG00000077039 | ||
| Gene (Danio rerio) | flna | ensemble: ENSDARG00000074201 | ||
| Gene (Danio rerio) | kdrl | ensemble: ENSDARG00000105215 | ||
| Gene (Danio rerio) | mmp14a | ensemble: ENSDARG00000002235 | ||
| Gene (Danio rerio) | msna | ensemble: ENSDARG00000058128 | ||
| Gene (Danio rerio) | rb1 | ensemble: ENSDARG00000006782 | ||
| Gene (Danio rerio) | s1pr1 | ensemble: ENSDARG00000042690 | ||
| Gene (Danio rerio) | tyr | ensemble: ENSDARG00000039077 | ||
| Gene (Danio rerio) | vegfaa | ensemble: ENSDARG00000103542 | ||
| Gene (Homo sapiens) | AAVS1 | HGCN:22 Adeno-Associated Virus Integration Site 1 | (Kotin et al., 1992) | |
| Gene (Sus scrofa) | ROSA26 | This paper | Porcine homolog of mouse ROSA26 safe harbor locus for transgene integration | |
| Strain, strain background (Escherichia coli) | NEB Stable Competent E. coli | New England Biolabs | C3040I | Electrocompetent Cells |
| Strain, strain background (Escherichia coli) | One Shot TOP10 Chemically Competent E. coli | Thermo Fisher/Invitrogen | C404010 | Electrocompetent Cells |
| Genetic reagent (Danio rerio) | WIK | Zebrafish International Resource Center | ZIRC:ZL84 | Wildtype strain of zebrafish |
| Genetic reagent (Danio rerio) | Tg(UAS:mRFP)tpl2 | Balciunas lab | Tg(miniTol2 < 14XUAS:mRFP, γCry:GFP>)tpl2 | Maintained in the lab of D. Balciunas (Balciuniene et al., 2013) |
| Cell line (Homo sapiens) | K562 | ATCC | ATCC:CCL-243 | chronic myelogenous leukemia cell line |
| Cell line (Sus scrofa) | Porcine fibroblast cell line | This paper | Recombinetics, Inc | |
| Transfected construct (Homo sapiens) | pAAVS1-MND:GFP | This paper | B. Moriarity lab | |
| Transfected construct (Sus scrofa) | pROSA26 UbC:GFP UgRNA | This paper | Recombinetics, Inc | |
| Recombinant DNA reagent | pT3TS-nCas9n | Wenbiao Chen | Addgene:46757 | Plasmid for in vitro synthesis of Cas9 mRNA |
| Recombinant DNA reagent | p494-2a-TagRFP-CAAX-SV40 | This paper | available from J. Essner lab; Deposited at Addgene | |
| Recombinant DNA reagent | pGTag-2A-TagRFP-CAAX-SV40 | This paper | available from J. Essner lab; Deposited at Addgene | |
| Recombinant DNA reagent | pGTag-2A-Gal4/VP16-βactin | This paper | available from J. Essner lab; Deposited at Addgene | |
| Recombinant DNA reagent | pGTag-2A-eGFP-SV40 | This paper | available from J. Essner lab; Deposited at Addgene | |
| Sequence-based reagent | This paper | PCR primers and oligos | See Supplementary file 1 | |
| Commercial assay or kit | pCR4 TOPO TA Cloning Kit | ThermoFisher/Invitrogen | ThermoFisher:K457502 | |
| Commercial assay or kit | Zero Blunt TOPO PCR Cloning Kit | ThermoFisher/Invitrogen | ThermoFisher:K2800J10 | |
| Commercial assay or kit | NEBNext Ultra II DNA Library Prep Kit for Illumina | New England Biolabs | NEB:E7645L | For MiSeq multiplex DNA sequencing |
| Software, algorithm | CRISPRScan | A. Giraldez lab | http://www.crisprscan.org/ | (Moreno-Mateos et al., 2015) |
| Software, algorithm | pGTag | This paper | http://genesculpt.org/gtaghd/ and https://github.com/Dobbs-Lab/GTagHD Wierson et al., 2019b copy archived at https://github.com/elifesciences-publications/GTagHD | short homology arm design |
| Software, algorithm | ICE | Synthego | Inference of CRISPR Edits (ICE)https://www.synthego.com/products/bioinformatics/crispr-analysis | Indel analysis of Sanger sequenced DNA |
| Software, algorithm | Cas-Analyzer | RGEN | CRISPR RGEN Tools http://www.rgenome.net/cas-analyzer/#! | Indel analysis of NextGen sequenced DNA |