FIG. 5.

FCS diffusion laws: (a) a compartmentalized cell membrane along with the underlying cytoskeleton. In the FCS diffusion law, the diffusion time at increasing observation areas is determined by fitting the respective autocorrelation functions. (b) The autocorrelation function for a small bin size decays rapidly when compared to an autocorrelation obtained from a larger area. (c) The transit time vs area is fitted using a linear function. The intercept value serves as the metric to distinguish between free diffusion, confined diffusion, and diffusion within a mesh.