Table 3.

Effects of various compounds on the activity of Nit09. Reactions were run for 2 min at 50 °C using 0.1 M citrate-phosphate buffer (pH 6.0), 1.25 μg purified protein, 5 mM phenylacetonitrile, and 1 mM putative inhibitor

Compound	Relative activity (%)a
Without additives	100.00 ± 0.64
EDTA	104.45 ± 1.62
DTT	76.21 ± 3.29
H2O2	24.56 ± 2.35
HgCl2	1.57 ± 0.36
AgNO3	1.96 ± 0.18
CaCl2	106.29 ± 2.71
MnSO4	98.01 ± 3.80
MgSO4	101.87 ± 5.92
FeSO4	98.25 ± 5.65
CuSO4	86.98 ± 3.82
CoSO4	81.01 ± 2.60
ZnCl2	114.00 ± 2.04
Sodium azide	98.89 ± 2.13
SDS	12.02 ± 2.77

^aActivity with phenylacetonitrile as substrate without additional additives (5.68 U/mg) was set as 100% relative activity