Table 1.

Binding constants for the BSA-ANS interaction in buffer and the ATPS at different pressures (T = 25 °C).

Solvent	Buffer	ATPS	ATPS
p/bar	aKb/M−1 106	bKb1/M−1 106	cKb2/M−1 106
1	4.2 ± 0.9	4.3 ± 2.0	0.42 ± 0.22
500	3.2 ± 1.1	5.4 ± 2.5	0.23 ± 0.12
1000	2.8 ± 0.9	0.41 ± 0.20	0.22 ± 0.12
1500	1.9 ± 0.6	0.82 ± 0.40	0.090 ± 0.045
2000	1.2 ± 0.4	0.40 ± 0.21	0.099 ± 0.050

^aThe binding stoichiometry for BSA:ANS is 1:3 at each pressure; ^bTwo sites were assigned for this class with stoichiometry 1:2 BSA:ANS; ^cOne site was assigned to this class with stoichiometry 1:1 BSA:ANS.