Table 1.
MISTiC experimental conditions.
| Experiment | Descriptor | Media switch (min) | Pre-culture condition | Test condition | Outliers |
|---|---|---|---|---|---|
| 1 | Q.S. (step response) | 105 | aTc | aTc + ara | 1;1;0;0 |
| 2 | Q.S. (step response, inverted) | 240 | aTc | aTc + ara | 4;0;1;2 |
| 3 | Q.S. (forced oscillator, 2 hr) | 140 | aTc | aTc ± ara | 1;1;1;5 |
| 4 | Q.S. (forced oscillator, 1 hr) | 184 | aTc | aTc ± ara | 0;0;1;3 |
| 5 | Distributed gene circuit oscillator | 217 | NA | IPTG | 0;0;3;1 |
| 6 | Auxotroph control | 218 | 0.25× AA + IPTG | 0.25× AA | 0;0;1;2 |
| 7 | Auxotroph (−F, −M) | 420 | 1× AA* + 0.1× F/M + IPTG | 1× AA* | 3;2;0;1 |
| 8 | Auxotroph (mixed) | 60 | 1× AA* + 0.1× F/M + IPTG | 1× AA* + IPTG | 5;2;2;1+ |
| 9 | Auxotroph (ΔmetA control) | 110 | 1× AA* + 0.1× F/M + IPTG | 1× AA* + IPTG | 1;2;3;8+ |
| 10 | Auxotroph (ΔpheA control) | 90 | 1× AA* + 0.1× F/M + IPTG | 1× AA* + IPTG | 3;2;2;7+ |
| 11 | Auxotroph (ΔmetA rescue) | 24 | 0.5× AA* + 1× M + 0.05× F + IPTG | 0.5× AA* + 1× M | 0;0;0;1 |
| 12 | Auxotroph (All AA) | 710 | 0.1× AA + IPTG | 0.003× AA | 1;0;0;0 |
| 13 | Auxotroph (All AA) | 148 | 0.1× AA + IPTG | 0.005× AA | 1;0;0;3 |
| 14 | Auxotroph (All AA) | 285 | 0.2× AA + IPTG | 0.02× AA | 1;2;0;0 |
Pre-culture conditions refer to the environment within the microfluidic device from the beginning of the experiment to the time of the first media switch. Test culture conditions refer to the media conditions following the first media switch. Quorum-sensing experiments were performed in Luria Broth (LB), whereas remaining experiments used MOPS EZ Rich Defined Medium, with the modifications specified above. AA refers to EZ amino acid solutions containing all amino acids and AA* refers to an amino acid solution lacking methionine (M) or phenylalanine (F). In experiments where F and M were added separately, 1× F and M refers to 0.4 mM and 0.2 mM, respectively. The pre-culture amino acid fraction was varied to control cell growth and fluorescent reporter expression. Outliers refer to the number of paired growth chambers that were excluded from the analysis out of n = 10 biological replicates for each interaction channel length (25 μm;50 μm;100 μm;250 μm) based on a set of specific criteria (Methods). +For these experiments, the numbers represent single outlier growth chambers that were excluded from the analysis out of n = 20 total biological replicates for 25 μm, 50 μm, 100 μm, and 250 μm interaction channels (Methods).