Fig. 3.

An overview of the process parameters measured during the production process of PLGA nanoparticles. a Manufacture—polymer recovery after manufacture of the PLGA 50:50, 75:25 and 85:15 nanoparticles formulated using microfluidics (MF) at TFR 10 mL/min and FRR 1:1, 3:1 and 5:1. b Purification via TFF—physicochemical characteristics before (MF) and after purification (TFF) and recovery of the polymeric nanoparticles (FRR 1:1, TFR 10 mL/min). c Monitoring—the particle size was monitored with Malvern OFF-line and AT-line in order to demonstrate the capability of the microfluidics method for continuous manufacturing of PLGA nanoparticles (particle sizes and intensity graphs for PLGA nanoparticles produced at FRR 1:1 and TFR 10 mL/min). For this study, nanoparticles without loaded protein were studied