Fig. 4.

Incorporation of proteins within the PLGA nanoparticles. The effect of the FRR on the physicochemical characteristics of the PLGA nanoparticles incorporating OVA (TFR 10 mL/min): a particle size and particle size distribution, b zeta potential and c encapsulation efficiency (% of calculated concentration divided by initial amount added and multiplied by FRR dilution factor; 0.2 mg/mL OVA initial concentration) vs loading (wt/wt%). Results represent mean ± SD of at least triplicate measurements. SEM micrographs of OVA-loaded d PLGA 50:50, e PLGA 75:25 and f PLGA 85:15 nanoparticles formulated at FRR 1:1 and TFR 10 mL/min after purification