Fig. 5.

Effects of glutamate and MK801 on the vessel-associated migration of GABA interneurons. a 3D Imaris modeling of migrating interneurons along radial microvessels in Gad67-GFP mice at P2. GFP-positive neurons were visualized by immunohistochemistry and microvessels labeled with CD31 antibodies. b–d Microphotographs acquired from the same cultured slice at different times of tracking (0 min, b; 60 min, c; and 150 min, d). Arrows indicate migrating cells along microvessels. Arrowheads indicate immobile large cells. e Stacked graph visualizing the position of several cells during 6-h video-tracking. f Quantification of the distance covered by the four cells identified in e. g Effects of glutamate (50 µM) and MK801 (20 µM) on the migration speed of cells moving tangentially and radially along microvessels. **p < 0.01 vs control and ^###p < 0.001 vs glutamate. The tests used for the statistical analysis, the number of independent experiments, the number of measures per experiment, and p values are detailed in Table 1