Table 2.

CRISPRi-based repression of plasmid-borne gfpUV gene in P. sonchi SBR5. Paenibacillus sonchi SBR5(pBV2mp-gfpUV) carried gfpUV coding sequence targeted by sgRNA-guided dCas9. GfpUV mean fluorescence intensity (MFI) of recombinant cells was determined by means of flow cytometry. Data represent means and standard deviations of technical triplicates. Different numbers represent significant differences by Scott-Knott test (p < 0.05)

	GfpUV MFI
SBR5(pBV2mp-gfpUV)(pNW33N)	1.49	±	0.33	(a2)
SBR5(pBV2mp-gfpUV)(piCas)	1.33	±	0.23	(a2)
SBR5(pBV2mp-gfpUV)(piCas-tgfpUV)	0.41	±	0.07	(a1)