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. 2020 May 15;11:2446. doi: 10.1038/s41467-020-16175-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Libraries of pathway elements such as promoters (bent arrow), RBSs (chord), coding sequences (arrow), terminators (“T”) are assembled to generate a combinatorial library, in which the microbial members produce different levels of the target metabolite. High-throughput techniques screen the library for the optimized pathway variant. Consequently, the best producer is used for large-scale production.