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. 2020 May 15;10:8105. doi: 10.1038/s41598-020-65014-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pretreatment with 14,15-EET increased the adhesiveness of HCAECs to THP-1 cells. HCAECs were untreated (A) or treated with vehicle (B), 0.25 nM (C), 2.5 nM (D), or 25 nM (E) 14,15-EET for 24 h. The amount of THP-1 cell adherence is represented as relative fluorescence units. Pretreatment with 0.25 nM, 2.5 nM, or 25 nM 5,6-EET significantly and dose-dependently increased the adhesiveness of HCAECs to THP-1 cells (P < 0.001) (F).