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. 2020 May 15;10:8024. doi: 10.1038/s41598-020-64895-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the homoplastic −47 A/T SNP on the expression of a downstream gfp gene. The promoter activity was expressed in relative fluorescence levels per unit of optical density (RFU; fluorescence level/OD) of GFP. The activity of promoter with T nucleotide at −47 was compared to the promoter with A nucleotide in M. smegmatis (a) and M. tuberculosis H37Ra (b). Mycobacterial cells carrying the pFPV2 plasmid served as a positive control for GFP expression, whereas cells without the plasmid served as the negative control. The presented data were averaged RFU from three independent experiments. Asterisks represent significant difference; p-value <0.001.