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. 2020 May 15;10:8060. doi: 10.1038/s41598-020-64950-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cross-talk between MEGF11 and IL-17A. MEGF11 was knocked down with short hairpin RNA (shRNA) and over-expressed with the pCMV-AC-GFP vector in TNBC cell line MDA-MB-231 and MDA-MB-468 cells. When the MEGF11 gene was knocked down, mRNA transcripts of IL-17A in ΔMEGF11 (a) and o/e MEGF11 (b) were quantified by real-time PCR (n = 4). After administration of different doses (0, 0.001, 0.1 ng/mL) of IL-17A in cultured media, MEGF11 protein (c) and IL-17A-related signalling proteins in MDA-MB-231 (d) and MDA-MB-468 (e) were analysed by Western blot (n = 4–6). The mRNA transcripts of MEGF11, IL-17A, and IL-17 receptors (IL17RB, IL17RC) in MDA-MB-231 and MDA-MB-468 cells (f) were quantified with real-time PCR (n = 6). Data between two groups were analysed with Mann-Whitney U or Student’s t test, while dose-related data were analysed with one-way ANOVA, followed by Dunnett’s post hoc test. Asterisks indicate a p value <0.05 compared to the wild type (for ΔMEGF11), the scramble group (for o/e MEGF11) or the vehicle group (for dose-dependent study).