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. 2020 May 15;10:8029. doi: 10.1038/s41598-020-64900-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluoroscopy of the micro-EMB device inside the left ventricle (LV), assessed from lateral (Lat) and posterioanterior (PA) views. Various areas of the anterior, septal, posterior and lateral ventricle wall can be sampled (a,b). (b) is magnified in panel (c) where the guide catheter (GC), housing the micro-catheter (MC), the flange of the micro-bioptome (F), and the device tip (DT) is seen (n = 6 animals). (d) Distribution of scores for all biopsy attempts (n = 157, n = 6 animals). Scores were visually assessed by microscopy upon retraction, using a subjective scale ranging from 0 (no sample) to 4 (large protruding sample). There was no difference in RNA-seq quality or overall gene expression signature between samples with a score of 2–4 (dark grey). Samples with a score of 0 or 1 were considered as failures (light grey).