Fig. 2. Over-expression of PAP1 is not sufficient to suppress the anthocyanin deficiency in tt19-8.

a PAP1 mRNA accumulation levels were evaluated by RT-qPCR and normalized to ACT2 between the various lines of RNA extracted from 4-day-old seedlings grown in AIC. n = 9, 7, 8, 7, 8, 8 biological replicates. b Anthocyanin levels from three independent experiments were measured by A₅₃₂ (normalized per mg of dry weight). The error bars represent the standard deviation of the average. Different letters indicate significant differences between the lines based on one-way ANOVA with Tukey’s Honest Significant Difference test (P < 0.05). Source data are provided as a Source Data file.