Table 4.
Experimental design for the optimization of the hydrolysis of coconut oil, using the best lipase cocktail (75% RML and 25% CALB)
| Run | Temperature (°C) | Biocatalyst content (%) | Molar ratio (water/oil) | Conversion (%) |
|---|---|---|---|---|
| 1 | 35 (−1) | 10 (−1) | 5:1 (−1) | 58.92 ± 1.10 |
| 2 | 35 (−1) | 10 (−1) | 10:1 (+ 1) | 61.49 ± 0.92 |
| 3 | 35 (−1) | 20 (+ 1) | 5:1 (−1) | 21.42 ± 0.83 |
| 4 | 35 (−1) | 20 (+ 1) | 10:1 (+ 1) | 60.87 ± 1.06 |
| 5 | 55 (+ 1) | 10 (−1) | 5:1 (−1) | 55.59 ± 0.69 |
| 6 | 55 (+ 1) | 10 (−1) | 10:1 (+ 1) | 62.54 ± 0.24 |
| 7 | 55 (+ 1) | 20 (+ 1) | 5:1 (−1) | 87.67 ± 0.93 |
| 8 | 55 (+ 1) | 20 (+ 1) | 10:1 (+ 1) | 96.16 ± 1.12 |
| 9 | 35 (−1) | 15 (0) | 7.5:1 (0) | 71.96 ± 0.77 |
| 10 | 55 (+ 1) | 15 (0) | 7.5:1 (0) | 81.40 ± 0.57 |
| 11 | 45 (0) | 10 (−1) | 7.5:1 (0) | 79.28 ± 0.66 |
| 12 | 45 (0) | 20 (+ 1) | 7.5:1 (0) | 98.68 ± 0.19 |
| 13 | 45 (0) | 15 (0) | 5:1 (-1) | 96.94 ± 0.85 |
| 14 | 45 (0) | 15 (0) | 10:1 (+ 1) | 87.15 ± 1.31 |
| 15 | 45 (0) | 15 (0) | 7.5:1 (0) | 78.53 ± 2.23 |
| 16(C) | 45 (0) | 15 (0) | 7.5:1 (0) | 78.70 ± 2.78 |
| 17(C) | 45 (0) | 15 (0) | 7.5:1 (0) | 79.28 ± 2.35 |
| 18(C) | 45 (0) | 15 (0) | 7.5:1 (0) | 79.66 ± 2.14 |
Further details are given on the “Materials and methodology” section