Fig. 3. Correlation of sensitivity with biosynthetic cost for heterotrophic or mixotrophic (HT and MX, a and b) and photoautotrophic (PA, b and d) growth conditions.

a, b Sensitivity values were grouped according to the classification of the biomass precursor, them being: amino acids (AA), carbohydrates (CB), triacylglycerols (TAG), all cis-sulfoquinovosyl diacylglycerol (As), sulfoquinovosyl diacylglycerols (Sqdg), monogalactosyl diacylglycerol (Mgdg), digalactosyl diacylglycerol (Dgdg), phosphatidylcoline (Pchol), phosphatidyl inositol (Pail), and phosphatidyl ethanolamine (Pe). As done for Fig. 1, we calculate relative activity as the ratio of the activity at each timepoint versus the initial activity to allow for comparisons across organisms (see activity in Methods). We identified the effect of molecular weight on sensitivity, as smaller molecules such as amino acids and carbohydrates with molecular weights from 137 to 238 g mol⁻¹ showed sensitivities at least 4 times lower than those of lipids, whose molecular weights range from 737 to 1080 g mol⁻¹. c, d Amino acids are sorted from smallest to biggest sensitivities. Included organisms are abbreviated as follows: Chlorella vulgaris (Cv), Phaeodactylum tricornutum (Pt), Saccharomyces cerevisiae (Sc), Yarrowia lipolytica (Yl), and Chinese Hamster Ovary cells (CHO).