Fig. 1.

a-aI Concentrations (mg/g) of fractionated lectins extracted from three leguminous seeds (fava bean, lentil, and pea). Lectin fractionation was obtained by precipitation with ammonium sulfate at 30, 70 and 90% saturations followed by extensive dialysis. Results are expressed as mean of three replicates with ± standard deviation. a-aII Concentrations (mg/g) of 90% fraction of lectins extracted from (fava bean, lentil, and pea) before and after purification by affinity chromatography. b-bI SDS-PAGE profiles of fractionated partially purified lectins extracted from three leguminous seeds after Coomassie blue staining. Lanes 1, 4, 7 represent 90% saturation. Lanes 2, 5, 8 represent 70% saturation. Lanes 3, 6, 9 represent 30% saturation. b-bII SDS-PAGE profiles and its electropherogram of purified lectins obtained by affinity chromatography after Coomassie blue staining. The purified lectins subunits bands appear at molecular weight 18, 14, 19 kDa of fava bean, lentil, and pea, respectively