Table 7.
Showed all data (PEAKS 8.5 software) of the complete amino acid sequences of lentil lectin
| Peptide sequences (this study) | − 10lgP | Mass | Length | ppm | m/z | RT | Scan | Intensity sample | Peptide location | PTM | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Start | End | ||||||||||
| K.NIENYGLAVLEIK.A | 43.18 | 1474.80 | 13 | 5.8 | 738.41 | 22.35 | 4388 | 7.66E3 | 124 | 136 | |
| K.ANAFLSPHHYDSEAILFNIK.G | 26.03 | 2286.14 | 20 | − 9.1 | 572.54 | 20.48 | 3985 | 0 | 137 | 156 | |
| K.VLQAALK.S | 23.74 | 741.47 | 7 | 9.7 | 371.75 | 12.84 | 2366 | 1.28E4 | 239 | 245 | |
| Total 3 peptides | |||||||||||
These peptide sequences were matched and compared using NCBI BLAST. The result of purified lentil lectin, in this study, showed high similarity (100%) to p54 protein that was found in pea
− 10lgP = Conversion form of P-value in PEAKS 8.5 software where P-value of 1% is equivalent to -10lgP of 20
ppm delta Mass is the deviation of measured mass from the theoretical mass of the peptide, in ppm, m/z mass of ions (in gas phase) per charge, RT relatively accurate prediction of peptide, PTM post translational modification