Table 8.
Showed all data (PEAKS 8.5 software) of the complete amino acid sequences of pea lectin (psl)
| Peptide Sequences (This study) | − 10lgP | Mass | Length | ppm | m/z | RT | Scan | Intensity sample | Peptide location | PTM | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Start | End | ||||||||||
| R.HIGIDVNSIK.S | 62.31 | 1094.61 | 10 | 12.0 | 365.88 | 15.58 | 2621 | 6.72E5 | 166 | 175 | |
| S.TETTSFLITK.F | 40.05 | 1139.61 | 10 | 1.7 | 570.81 | 16.93 | 2848 | 6.15E3 | 31 | 40 | |
| R.ALYSSPIHIWDR.E | 38.29 | 1456.75 | 12 | 7.1 | 486.59 | 19.30 | 3256 | 7.71E3 | 74 | 85 | |
| R.HIGIDVN (+ 0.98) SIK.S | 38.07 | 1095.59 | 10 | 10.9 | 366.21 | 15.19 | 2553 | 1.31E4 | 166 | 175 | Deamidation (NQ) |
| K.FSPDQQNLIFQGDGYTTK.E | 34.19 | 2057.97 | 18 | 2.4 | 686.99 | 18.74 | 3159 | 0 | 41 | 58 | |
| D.RHIGIDVNSIK.S | 32.44 | 1250.71 | 11 | 4.1 | 417.91 | 14.28 | 2399 | 1.55E5 | 165 | 175 | |
| K.DVVPEWVR.I | 30.86 | 998.52 | 8 | 7.2 | 500.27 | 18.34 | 3090 | 1.13E4 | 231 | 238 | |
| Total 7 peptides | |||||||||||
| Peptide sequence of (previous study) | Peptide location (β chain) | References |
|---|---|---|
| HIGIDVNSIK | 136–145 | Sharon and Lis (1990) |
| TETTSFLITK | 1–9 | |
| ALYSSPIHIWDR | 42–53 | |
| FSPDQQNLIFQGDGYTTK | 10–27 | |
| PEWVR | 200–204 | |
| DVVPEWVR | 83–90 | Hemperly et al. (1979) |
− 10lgP = Conversion form of P-value in PEAKS 8.5 software where P-value of 1% is equivalent to − 10lgP of 20
ppm delta Mass is the deviation of measured mass from the theoretical mass of the peptide, in ppm, m/z mass of ions (in gas phase) per charge, RT relatively accurate prediction of peptide, PTM post translational modification, NQ variable modification deamidation NQ (+ 0.98 Da) of asparagines (N) and glutamines (Q)