Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 15;10:93. doi: 10.1186/s13568-020-01025-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Typical cell line development (CLD) workflow change following media development (a) traditional CLD using classical media (with serum) during cell line screening and then adapting to (serum-free) commercial media, ((b) recent CLD changes by adapting host cells to commercial media and using throughout the CLD process, however transfection and cloning requires a unique media, while production will mostly require a rich production media (recent commercial media) to achieve higher productivities, c our intended CLD method in this study which uses a rich production media (recent commercial media) as platform media throughout the study minimizing use of different media from the platform media

Fig. 1 — Typical cell line development (CLD) workflow change following media development (a) traditional CLD using classical media (with serum) during cell line screening and then adapting to (serum-free) commercial media, ((b) recent CLD changes by adapting host cells to commercial media and using throughout the CLD process, however transfection and cloning requires a unique media, while production will mostly require a rich production media (recent commercial media) to achieve higher productivities, c our intended CLD method in this study which uses a rich production media (recent commercial media) as platform media throughout the study minimizing use of different media from the platform media