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. 2020 May 15;10:8056. doi: 10.1038/s41598-020-64841-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Percentage and count of ARG1-expressing cells in different immunophenotypes of circulating-MDSCs. (a) Flow cytometry chart of ARG1-expressing MDSCs in the peripheral blood of gastric cancer patients (GC-PB). Cells were first gated based on the expression of HLA-DR. Subsequently, the HLADR ^-/low population were gated based on the co-expression of ARG1 and CD11b, ARG1 and CD14, ARG1 and CD15, ARG1 and CD33; the CD33⁺ARG1⁺ cells were gated based on the expression of CD14 and CD15. An isotype control was also run (Supplementary Fig. 1). (b) Flow cytometry chat of ARG1-expressing cells in the peripheral blood of healthy donors (HD-PB). Flow cytometry showing different MDSC phenotypes in GC-PB or HD-PB. An isotype control was also run (Supplementary Fig. 1). (c) Gated on HLA-DR^-/low cells, the percentage and count of ARG1-expressing cells in CD33⁺ or CD14⁺ or CD15⁺ or CD11b⁺ MDSC subsets of peripheral blood. (d) Gated on HLA-DR^-CD33⁺MDSCs, the percentage and count of ARG1-expressing cells in CD14⁺CD15^- or CD14⁺CD15⁺ or CD14^-CD15⁺ or CD14^-CD15^- MDSC subsets of peripheral blood. Values were mean ± SEM (n = 20), **p < 0.01. Paired /Wilcoxon matched-pairs signed rank test or unpaired/Mann–Whitney tests. Abbreviations: P11, population11 (CaliBRITE fluorescent microsphere).