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. 2020 May 15;11(5):372. doi: 10.1038/s41419-020-2572-3

Fig. 1. Circ-DAB1 was upregulated during BMSC osteogenic differentiation.

Fig. 1

a qRT-PCR validated the five most upregulated circRNAs in BMSCs during osteogenic differentiation at day 7 day versus day 0. b Schematic picture of the back-splicing of circ-DAB1 from DAB1. c Agarose gel electrophoresis corroborated the existence of circ-DAB1 in PCR products of divergent primers in cDNA but not gDNA in BMSCs. d qRT-PCR and northern blot of circ-DAB1, linear DAB1 mRNA, and GAPDH in BMSCs treated without or with RNase R. e, f FISH and subcellular fractionation detected the distribution of circ-DAB1. Scale bar: 30 μm. **P < 0.01. Experiments conducted with three biological repeats.