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. 2020 May 15;11(5):372. doi: 10.1038/s41419-020-2572-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a qRT-PCR of circ-DAB1 level in untreated BMSCs (left) or BMSCs cultured for 7 days in osteogenic media (right) under the transfection of pcDNA3.1(+)/circ-DAB1 (circ-DAB1 group) versus vector (NC group) or sh-circ-DAB1#1/2 versus sh-NC. b CCK-8 analysis measured the proliferation of BMSCs or OS cell model following indicated transfections. c ALP and ARS staining in BMSCs or OS cell model following specific transfections. Scale bar: 100 μm. d, e qRT-PCR detection and western blots of the mRNA and protein of osteogenic markers in BMSCs or OS cell model following specific transfections. **P < 0.01. Experiments conducted with three biological repeats.