Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 15;11(5):372. doi: 10.1038/s41419-020-2572-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Luciferase activity of DAB1 promoter in response to circ-DAB1-overexpressing plasmids. b, c The interaction between circ-DAB1 and RBPJ was probed with RIP and RNA pull-down assays. d, e ChIP and DNA pull-down assays assessed the interaction between DAB1 promoter and RBPJ. f qRT-PCR analysis of the enrichment of DAB1 promoter in RBPJ ChIP products in BMSCs under circ-DAB1 overexpression. g qRT-PCR and western blots of RBPJ mRNA and protein levels in BMSCs with circ-DAB1 overexpression. h, i qRT-PCR and western blot of RBPJ level in BMSCs transfected with pcDNA3.1/RBPJ (RBPJ group) versus pcDNA3.1 (NC group) or with sh-RBPJ#1/2 versus sh-NC. j qRT-PCR of circ-DAB1 expression in BMSCs with RBPJ overexpression or knockdown. k qRT-PCR and western blot of DAB1 level in BMSCs with RBPJ overexpression or knockdown. **P < 0.01. n.s. indicated no significance. Experiments conducted with three biological repeats.