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. 2020 May 15;5:40. doi: 10.1038/s41541-020-0185-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Groups of 4-weeks-old (subadult group) and 1-day-old (juvenile group) chickens were a immunized twice with R65_mono/H17N10 MLIV candidate and b challenged with a homologous HPAIV. Five naive contact controls (sentinels) accompanied each group. Three naive controls served as negative-control and from d23 on as positive control to verify the challenge infection.