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. 2020 May 15;11(5):374. doi: 10.1038/s41419-020-2590-1

Fig. 3. Myeloid cell-specific BIG1 KO protected mice from LPS-induced endotoxemic shock.

Fig. 3

WT and myeloid cell-specific BIG1 KO (BIG1 cKO) mice were intra-peritoneally administered with either LPS (50 mg/kg) or isometric saline. a Survival curves of LPS-treated WT and BIG1 cKO mice were calculated according to the Kaplan–Meier method. Survival analysis was performed using log-rank test (**P < 0.01; n = 10). b Body temperature of WT and BIG1 cKO mice were measured at the indicated time period. Mean ± SEM is presented and analyzed by Student’s t-test (***P < 0.001; n = 6). c Body weight loss of WT and BIG1 cKO mice were measured at the indicated time period. Mean ± SEM is presented and analyzed by Student’s t-test (***P < 0.001; n = 6). d The mRNA levels of the indicated cytokines in peritoneal macrophages from WT and BIG1 cKO mice injected intra-peritoneally with LPS (50 mg/kg) were measured by RT-qPCR at the indicated time period. Mean ± SEM is presented and analyzed by Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001; n = 6). e Blood was collected from WT and BIG1-cKO mice injected intra-peritoneally with LPS (50 mg/kg) at the indicated time points. Serum levels of the indicated cytokines from WT and BIG1 cKO mice were measured by ELISA at the indicated time point. Mean ± SEM is presented and analyzed by Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001; n = 6). f, g Liver and lung sections from WT and BIG1 cKO mice were subjected to H&E staining at the indicated time point. The representative images were shown (original magnification, ×400;Scale bars, 50 μm). The inflammatory infiltration score was quantified. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001; n = 6; Student’s t-test.