Table 1.
Cryo-EM data collection, refinement, and validation statistics.
| HsLRRC8D (EMD-30029) (PDB 6M04) | |
|---|---|
| Data collection and processing | |
| Magnificationa | x23,500 |
| Voltage (kV) | 200 |
| Electron exposure (e–/Å2) | 50 |
| Defocus range (μm) | 0.5–2.5 |
| Pixel size (Å) | 1.490 |
| Symmetry imposed | C2 |
| Initial particle images (no.) | 2,056,807 |
| Final particle images (no.) | 247,154 |
| Map resolution (Å) | 0.143 |
| FSC threshold | |
| Map resolution range (Å) | 3.9–6.0 |
| Refinement | |
| Initial model used (PDB code) | 5ZSU, 6G9O |
| Model resolution (Å) | 0.5 |
| FSC threshold | |
| Model resolution range (Å) | 4.36 |
| Map sharpening B factor (Å2) | −211.2 |
| Model composition | |
| Non-hydrogen atoms | 34,666 |
| Protein residues | 4282 |
| B factors (Å2) | |
| Protein | 106.9 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.007 |
| Bond angles (°) | 1.288 |
| Validation | |
| MolProbity score | 2.38 |
| Clashscore | 10.11 |
| Poor rotamers (%) | 2.06 |
| Ramachandran plot | |
| Favored (%) | 87.11 |
| Allowed (%) | 12.85 |
| Disallowed (%) | 0.05 |
aCalibrated pixel size at the detector.