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. 2020 May 15;10:8090. doi: 10.1038/s41598-020-64891-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design of the attenuation-compensated Airy light sheet microscope, and Airy beam profiles. (a) Experiment setup with attenuation-compensation provided by the NDF. (b–d) show the 2 + 1D Airy light-sheet profiles attenuated by an absorbing NIR dye (2 mM) and visualized in fluorescein to show two-photon fluorescence with (b) no attenuation-compensation, (c) attenuation-compensated by the NDF, and (d) attenuation-compensated by the SLM ( $σ = 0.5$ ). (e) Normalized peak transverse intensity versus propagation distance corresponding to beam profiles in (b–d). CAM: Camera, CL: Cylindrical Lens, CPM: Cubic Phase Mask, GM: Galvo Mirror, L: Lens, NDF: Neutral Density Filter, O: Objective, SC: Sample Chamber, SLM: Spatial Light Modulator, TL: Tube Lens. The $x, y, z$ coordinate system shown in (a) applies within the sample volume only.