Figure 6.

Maximum intensity projections of deconvolved single-photon Airy LSFM images (a–c) of ~$450$ μm diameter SH-SY5Y spheroid stably expressing GFP. (a) No attenuation-compensation, (b) attenuation-compensation using NDF, and (c) attenuation-compensation using SLM ($\sigma =0.8$). (d) Intensity profiles along line (1) near the start of the FOV where intensities of all three cases match and (e) intensity profiles along line (2), ~$150\mu m$ deeper into the sample. (f) SNR plotted as a function of propagation coordinate, $x$, for no-attenuation compensation, attenuation-compensation using NDF, and attenuation-compensation using SLM ($\sigma =0.8$). (g) Ratios of SNR with NDF-based and SLM-based compensations compared to the case of no compensation. The arrow in (a) denotes the direction of propagation of the Airy light-sheet. Look-up tables of the images shown in (a–c) are independently scaled to the data shown. Line intensity profiles in (d,e) are normalized with respect to the local mean background ${\mathrm{\mu }}_{\mathrm{b}}$. This dataset can be accessed at²¹.