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. 2020 May 15;10:8090. doi: 10.1038/s41598-020-64891-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ratios of SNR with NDF-based compensation and SLM-based compensation to no compensation of (a,b) deconvolved single-photon Airy LSFM image stacks of ~450 μm diameter SH-SY5Y spheroid and (c,d) recorded two-photon Airy LSFM image stacks of ~450 m diameter HEK-293 spheroid. (a,b) Data 1 - complete stack, Data 2–15 μm subset of complete stack at $z = 75 μ m$ , Data 3–15 μm subset of complete stack at $z = 90 μ m$ , and Data 4–20 μm subset of complete stack at $z = 105 μ m$ from the start of the stack. (c,d) Data 1 - complete stack, Data 2–15 μm subset of complete stack at $z = 25 μ m$ , Data 3–15 μm subset of complete stack at $z = 40 μ m$ , and Data 4–55 μm subset of complete stack at $z = 105 μ m$ from the start of the stack. This dataset can be accessed at this website²¹.