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. 2020 May 15;11:2420. doi: 10.1038/s41467-020-16214-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Volcano plot illustrating differentially expressed genes in GCTM-2^highCD9^highEPCAM^high versus general (unsorted) populations. b Principal component analysis of single-cell RNA-seq data on GCTM-2^highCD9^highEPCAM^high, GCTM-2^highCD9^high, GCTM-2^midCD9^mid, and GCTM-2^lowCD9^low subpopulations. c Joint species principal component analysis of single-cell RNA-seq data on GCTM-2^highCD9^highEPCAM^high (HHH), GCTM-2^highCD9^high (HH), GCTM-2^midCD9^mid (MID), and GCTM-2^lowCD9^low (LOW) subpopulations alongside cynomolgus embryo data from ref. ³⁰; single embryo cells classified according to Houghton et al.³⁰. Top left: screeplot demonstrating the amount of variability in the data accounted for by each component; top right: graph displaying data distribution along first and second components; bottom left: graph displaying data distribution along the second and third components. Color and shape of point indicate sample phenotype, each point representing a single cell.