Table 4.

Estimation of the impact of the breadth of target and sensitivity of platforms at two different mutant allele fraction levels in the detection of KRAS mutations in a mCRC population.

	Prevalence of KRAS mutations in mCRC	Breadth of KRAS targets	Sensitivity at 50 ng DNA mAF 0.50%a	Sensitivity at 50 ng DNA mAF 0.02%a	Estimated % of KRAS positive patients detected (50 ng, mAF 0.50%)	Estimated % of KRAS positive patients detected (50 ng, mAF 0.02%)
ddPCR	46%	82%	100%	58%	38%	22%
Idylla	46%	96%	50%	8%	22%	4%
COBAS z480	46%	96%	38%b	0%	17%	0%
BEAMing	46%	94%	75%c	25%d	32%	16%

^aSensitivity was based on the detection of KRAS p.G12 and p.G13 mutations in synthetic reference samples at the indicated input and mAF.

^bInvalid result were obtained for 25% of the results.

^cInvalid result were obtained for 25% of the results.

^dInvalid result were obtained for 12% of the results.