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. 2020 May 15;11(5):377. doi: 10.1038/s41419-020-2565-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The co-localization of C3d (red) and GFAP (green) was assessed by confocal microscopy. b The percentage of GFAP-positive astrocytes that were C3d-positive in the hippocampus DG region. c The quantification of GFAP-positive astrocytes in the hippocampus DG region. Field: 70 × 70 μm. d Western blots and densitometric analysis for C3d, GFAP, and S100A10. β-actin was used as an internal control. The data are the mean ± SEM, n = 6 biologically independent animals, *P < 0.05, **P < 0.01, ***P < 0.001.