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. 2020 May 15;11(5):377. doi: 10.1038/s41419-020-2565-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a A schematic diagram of primary astrocytes treatment with IL-18. b qPCR analysis of cultured astrocytes treated with IL-18 (A1 marker: H2-T23, Fkbp5 and ligp1; A2 marker: Emp1, S100A10, and Cd109). c Western blots and densitometric analysis for C3d, p-p65, and p65. The relative expression of C3d normalized to β-actin and the relative expression of p-p65 normalized to p65. d Immunofluorescence for C3d (green) and p65 (red) in cultured astrocytes. The data are the mean ± SEM, n = 6, *P < 0.05, **P < 0.01, ***P < 0.001.