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. 2019 Nov 15;22(5):625–638. doi: 10.1093/neuonc/noz214

Fig. 2.

Fig. 2

TAA secreted CCL2 in disseminated MB. (A) ELISA-detected cytokines in CM of TAA isolated from Group 4 MB7609, Group 3 MB7965, and Group 3 MB9678. (B) Concentration of CCL2 in CM of TAA (n = 4) and normal astrocytes (n = 3). (C) Concentration of CCL2 in supernatant of disseminated and primary TAA culture (n = 3). (D) Expression of CCL2 mRNA in TAA, MBSCs, and MB cells derived from MB (n = 6, 2 WNT, 2 sonic hedgehog and 2 Group 3 MB). (E) Immunoblotting analysis of CCL2 in disseminated MB and CB. (F) CCL2 mRNA expression in disseminated MB examined by in situ hybridization. (G) CCL2 mRNA expression in 9-month, 3-year, and 42-year CB examined by in situ hybridization. (H) CCL2 mRNA expression in isolated astrocytes from 9-month CB. Scale bars, 10 μm. Abbreviation: AS, astrocytes.