Figure 1.

Bacterial Composition in Mock Community

(A) Relative abundance of the 20 mock bacteria across the dilutions as identified by sMETASeq. Contaminants are indicated in light and dark gray.

(B) Similar as in (A) for 16S rDNA-seq.

(C) Correlation of mock bacteria abundance between sMETASeq and 16S rDNA-seq for pooled samples. The Pearson's correlation values for each individual bacterium across dilutions are indicated with color. p = 0.00057. See also Table S2.

(D) Number of correctly assigned mock species for sMETASeq (light blue) and 16S rDNA-seq (dark blue) across dilutions. Dilutions D1–D8 have the same number of correct assignments and are therefore pooled.

(E) Comparison of diversity measurements between sMETASeq and 16S rDNA-seq across the dilutions.