Table 1.
Composition of each solution and the time and temperature of each step.
| DMSO (v/v%) | EG (v/v%) | COOH-PLL (w/v%) | Sucrose (mol) | Time (min) | Temperature | |
|---|---|---|---|---|---|---|
| Solutions for pre-vitrification treatment | ||||||
| Equilibration solution-1 | 10 | 10 | – | – | 5 | RT |
| Equilibration solution-2 | 10 | 10 | – | – | 12.5 | RT |
| Vitrification solution-1 | 20 | 20 | 10 | 0.5 | 5 | IT |
| Vitrification solution-2 | 20 | 20 | 10 | 0.5 | 7.5 | IT |
| Solutions for post-vitrification treatment | ||||||
| Rewarming solution | – | – | – | 1 | 1 | RT |
| Dilution solution | – | – | – | 0.5 | 3 | RT |
| Washing solution-1 | – | – | – | – | 5 | RT |
| Washing solution-2 | – | – | – | – | 5 | RT |
COOH-PLL: Carboxylated poly-l-lysine; DMSO: Dimethyl sulfoxide; EG: Ethylene glycol; IT: Ice-cold temperature; RT: Room temperature.