Fig. 2.

Representative scanning electron microscopic images showed a porous morphology of the chitosan-cartilage ECM composite scaffold with an average pore size of 75.8 ± 18.0 μm. (A) low-power view, bar = 500 μm. (B) high-power view, bar = 200 μm. Confocal microscopic images of chitosan-cartilage composite scaffolds after cell seeding. The scaffolds were stained yellow, while the viable ASCs were stained green. (C) Day 2 image showed multiple small ASC spheroids formation. (D) Day 5 image demonstrated further aggregation of the ASC spheroids.