Table 5.
CCD experimental design and the responses. The data are the average of at least three experiments ± standard deviation
| Run | X1 | X2 | X3 | Extract D.W. (mg/l) | Cell Death HepG2 (%) | Cell Death A549 (%) | Response A549 (%) |
|---|---|---|---|---|---|---|---|
| 1 | −1 | −1 | −1 | 66.7 | 11.5±4.9 | 15.6±3.5 | 5.8 |
| 2 | +1 | −1 | −1 | 93.4 | 96.5±4.8 | 98.3±1.7 | 51.3 |
| 3 | −1 | +1 | −1 | 70.0 | 99.3±4.8 | 97.4±2.5 | 38.2 |
| 4 | +1 | +1 | −1 | 93.4 | 95.3±5.6 | 98.5±1.3 | 48.8 |
| 5 | −1 | −1 | +1 | 106.7 | 46.9±2.0 | 40.2±4.5 | 23.9 |
| 6 | +1 | −1 | +1 | 158.9 | 28.4±4.4 | 23.1±4.8 | 20.5 |
| 7 | −1 | +1 | +1 | 76.7 | 56.5±3.9 | 50.8±3.4 | 21.8 |
| 8 | +1 | +1 | +1 | 178.8 | 36.1±0.2 | 32.8±1.5 | 32.8 |
| 9 | −1.73 | 0 | 0 | 55.6 | 32.6±3.9 | 27.5±2.0 | 17.3 |
| 10 | +1.73 | 0 | 0 | 122.2 | 77.26±0.9 | 64.2±1.1 | 43.9 |
| 11 | 0 | −1.73 | 0 | 91.2 | 38.5±2.0 | 37.4±4.8 | 19.0 |
| 12 | 0 | +1.73 | 0 | 126.7 | 80.03±2.8 | 70.2±0.2 | 49.7 |
| 13 | 0 | 0 | −1.73 | 83.3 | 51.6±5.3 | 49.9±14.2 | 23.0 |
| 14 | 0 | 0 | +1.73 | 171.2 | 19.02±0.6 | 16.2±7.3 | 15.5 |
| 15 | 0 | 0 | 0 | 126.7 | 97.7±3.8 | 99.2±0.1 | 70.3 |
| 16 | 0 | 0 | 0 | 130.0 | 98.9±1.7 | 95.4±4.2 | 69.4 |
| 17 | 0 | 0 | 0 | 126.7 | 95.0±1.4 | 97.3±2.6 | 68.9 |
| 18 | 0 | 0 | 0 | 124.5 | 99.9±5.4 | 97.4±3.3 | 67.8 |
| 19 | 0 | 0 | 0 | 135.6 | 97.3±0.9 | 97.0±2.0 | 73.6 |
| 20 | 0 | 0 | 0 | 144.4 | 93.8±4.5 | 97.5±3.0 | 78.7 |
CCD: Central composite design, D.W.: Dry weight.